Data on production of mammalian stable cells expressing secretory BEFV transmembrane deleted G protein

Expression of Biologically Active Recombinant B-Domain-Deleted Human Factor VIII in Mammalian Cells

Improved Protein Production from Mammalian Cells

There is a general consensus that although non-mammalian systems can be effectively employed in the manufacture of many proteins, mammalian systems will have to be chosen for the bulk production of a large number of proteins to be used in therapeutics. The main reason being that only the latter type of system can assure the correct form of post-translational modification that many proteins requ...

Subcellular localization of the transmembrane inner ear (Tmie) protein in a stable Tmie-expressing cell line

Mutations in the transmembrane inner ear (Tmie) gene, which encodes the Tmie protein, have been attributed to deafness autosomal recessive 6 (DFNB6), an autosomal nonsyndromic recessive hearing loss disorder. Although the Tmie gene was identified a few years ago, little is known about subcellular localization of the Tmie protein. In order to address this, we developed a stable cell line express...

Genetically Engineered Mesenchymal Stem Cells Stably Expressing Green Fluorescent Protein

Objective(s) Mesenchymal stem cells (MSCs) are nonhematopoietic stromal cells that are capable of differentiating into and contribute to the regeneration of mesenchymal tissues. Human mesenchymal stem cells (hMSCs) are ideal targets in cell transplantation and tissue engineering. Enhanced green fluorescent protein (EGFP) has been an important reporter gene for gene therapy. The aim of this stu...

G-Protein Coupled Receptor Signaling Architecture of Mammalian Immune Cells

A series of recent studies on large-scale networks of signaling and metabolic systems revealed that a certain network structure often called "bow-tie network" are observed. In signaling systems, bow-tie network takes a form with diverse and redundant inputs and outputs connected via a small numbers of core molecules. While arguments have been made that such network architecture enhances robustn...